Are you intrigued by how protein complexes enable plant cells to swiftly integrate environmental signals? If you have a strong passion for molecular biology, protein biochemistry, and quantitative bio-imaging, this PhD position could be a perfect fit for you. We are seeking a dedicated candidate to delve into the biochemical foundations of rapid signal integration in plants.

About the Company

The University of Amsterdam (UvA) is a vibrant institution located at the Amsterdam Science Park. The Swammerdam Institute for Life Sciences (SILS) is part of UvA's Faculty of Science, conducting high-quality life science research and offering education across various programs. With around 240 employees, SILS collaborates closely with industries in medical, biotech, chemical, and agricultural fields, focusing on themes like Cell & Systems Biology and Green Life Sciences.

The Role

You will be part of an NWO VIDI-funded project led by Dr. André Kuhn, investigating how plant cells rapidly integrate osmotic, salinity, and pathogen-derived signals. Your specific project will focus on B4-RAF protein kinases, which act as intracellular signal integrators activated by various environmental stimuli. These kinases form distinct subcellular puncta, suggesting they assemble into signaling complexes (signalosomes) that coordinate early cellular responses.

Responsibilities

• Identify and validate components of B4-RAF signaling complexes in Arabidopsis thaliana.
• Establish how these complexes assemble and localize under different environmental conditions.
• Determine the role of these complexes in stress resilience.
• Utilize techniques including immunoprecipitation coupled with tandem mass spectrometry, FRET-FLIM, co-immunoprecipitation, and live-cell confocal imaging.
• Generate and characterize Arabidopsis loss-of-function and fluorescent reporter lines.
• Conduct microfluidics-coupled live-cell imaging to study the dynamics and localization of B4-RAF signaling complexes.
• Integrate proteomics, imaging, and genetic datasets to develop mechanistic models of rapid plant signal integration.

Requirements

• An MSc degree in molecular biology, biochemistry, or a related field.
• Hands-on laboratory experience in at least two of the following areas: protein biochemistry, (plant) molecular biology, confocal microscopy, proteomics, and protein-protein interaction assays.
• Ability to plan experiments, maintain high-quality records, analyze results critically, and troubleshoot.
• Fluent in English, both written and spoken.
• Independent work capability and constructive collaboration in an international team.
• Experience with CRISPR/Cas9 genome editing in plants or analysis of proteomics and/or imaging data is a plus.

What We Offer

• A temporary contract for 38 hours per week for 4 years, with an initial 18-month evaluation period.
• A salary ranging from €3,204 to €4,051 gross per month, excluding holiday pay and year-end bonus.
• 232 vacation hours per year, parental leave, partner leave, and social engagement leave.
• Opportunities for professional development through training courses and open lectures.
• Assistance with relocation, including support for daycare and schooling for families moving to the Netherlands.

Application Process

If you believe you fit this profile and are interested in the position, we look forward to your application. Applications will be accepted until October 9, 2026. Please submit a detailed CV, a motivation letter, and the contact information for two references in a single PDF file. A knowledge security check may be part of the selection process.

The UvA is committed to diversity and inclusion, encouraging applications from all qualified individuals, particularly those from underrepresented groups.